For the ionization of intact macromolecules, the use of soft ionization techniques such as malditof ms and esi tof ms is necessary. He is head of the polymer analysis group at the bam and a member of the. Mass spectrometry of intact synthetic polymers has been limited to field desorption fd ms for many years. Characterization of synthetic polymers via matrix assisted. There are many techniques that can be employed in the characterization of synthetic homopolymers, but few provide as useful of information for end group analysis as matrixassisted laser desorption ionization time of flight mass spectrometry malditof ms. These advantages include low acquisition and operating costs, ease of use, ruggedness, and high throughput. Analysis of polymer additives in synthetic polymers using. Malditof mass spectrometry of synthetic polymers springer laboratory kindle edition by pasch, harald, schrepp, wolfgang. Analysis of additives in synthetic polymers is therefore important for the evaluation of improvement of polymer properties. Uvmatrixassisted laser desorptionionization timeof.
Maldi timeofflight mass spectrometry of synthetic polymers. A surfacelayer matrixassisted laser desorption ionization mass spectrometry imaging technique slmaldimsi is developed to study the chemical composition of multicomponent polymer surfaces. Malditof mass spectrometry of synthetic polymers harald. Malditof mass spectrometry is a valuable tool for the analysis of synthetic polymers polymer analysis software can be used to facilitate the interpretation of maldi data derived from polymer samples. Polymer analysis software for the characterisation of polymers and co polymers using maldi data. These instruments have recently being utilized more and more.
Recently, malditof ms has been used for the analysis of polymer additives. Sandler mass spectrometry users group university of california san francisco may 20, 2003 features of malditof ms soft ionization analyze intact biomolecules and synthetic polymers broad mass range analyze a wide variety of biomolecules simple mixtures are okay relatively tolerant of buffers and salts fast data acquisition easy to use and maintain, no water. To gain information on the single end group the oligomeric species have to be selected and fragmented in a cid process. Malditof mass spectrometry is an invaluable analytical tool for polymer characterization because of its ability to generate polymer ions in the singly charged state and with minimal fragmentation. The list of compounds analyzed by matrixassisted laser desorption ionization maldi mass spectrometry has grown steadily, and the technique has become a viable tool for detailed characterization of synthetic polymers.
Characterization of synthetic polymers via matrix assisted laser desorption. His particular interest is focused on the application of malditof ms of synthetic polymers by developing suitable hyphenated chromatography approaches for efficient reduction of complexity. Mass spectrometry of synthetic polymers analytical chemistry. This understanding is especially important when characterizing synthetic polymers by malditofms, due to the breadth of the polymer molecular mass distribution mmd. Using a polymer probe characterized by malditofms to assess. Analysis of synthetic polymers by malditof ms polymer analysis software for the characterisation of polymers and co polymers using maldi data automatically generate useful information e.
As the wellknown soft ionization techniques, matrix assisted laser desorptionionization maldi time of flight tof mass spectrometry and electrospray ionization esi ms are widely used in the investigative field of macromolecules including biomolecules and synthetic polymers since they were introduced in the late 1980s. Pdf in recent years, matrix assisted laser desorptionionization timeofflight malditof mass spectroscopy has become a routine analytical. Malditof mass spectral characterization of polymers. One of the most valuable tools for the characterization of polymers is matrixassisted laser desorption ionization time of flight mass spectrometry malditof ms, which provides a means to determine the m n and pdi of a polymer sample, as well as to accurately calculate the repeat unit and end group masses. In recent years, matrix assisted laser desorptionionization timeof. Investigating the effect of mixing ratio on molar mass. Nextgeneration sequencing and maldi mass spectrometry in. Mass spectrometry is a powerful technique that has been used to identify bacteria by their protein content and to assess bacterial functional traits through analysis of their specialized metabolites. Quantitative analysis of polymer additives with malditof. His research is driven from a regulatory perspective having strong ties to academia and industry. It has been applied to the analysis of biomolecules biopolymers such as dna, proteins, peptides and sugars and large organic molecules such as polymers, dendrimers and other. Malditof mass spectrometry of synthetic polymers springerlink.
A protocol for the matrixassisted laser desorption ionization time of flight mass spectrometry malditof ms characterization of synthetic polymers is described including the optimization of sample preparation, spectral acquisition, and data analysis. In the past, mass spectrometry of synthetic polymers was hardly possible. The results obtained by using norharmane, gentisic acid ga and other uvmaldi matrices, in positive and negative ion modes, were compared. Some polymers, such as polyethylene glycols and polymethylmethacylate form ions with alkali metals added to the sample or simply.
Malditof mass spectrometry molecular weight measurement of glycoproteins using a benchtop malditof mass spectrometer laanatme044 malditof mass spectrometers are a mass spectrometer type that is used in a wide range of fields likewise lcms, in terms of high throughput and high sensitivity. Malditofms, originally developed to study peptides and proteins, has also been successfully applied to synthetic polymers. Malditof ms is used for the qualitative analysis of seven different polymer additives directly from the polymer without tedious sample pretreatment. During the past decade, mass spectrometry has revolutionized the characterization of synthetic polymers1.
Mass spectrometry of synthetic polymers acs publications. Malditof mass spectrometry of synthetic polymers researchgate. Journal of the american society for mass spectrometry, jan 2005. This soft ionization technique utilizes short laser pulses to desorb solidstate samples of the polymer analyte embedded in a matrix compound to.
The amount of additive depends on the intended use of the polymer but, typically, it is less than 1% ww. Mass spectrometry in polymer chemistry wiley online books. Mass spectrometry is an analytical technique in which samples are ionized into charged molecules and ratio of their mass tocharge mz can be measured. Malditof mass spectrometry is one of the latest and most fascinating new.
Maldi of synthetic polymers nih nigms biomedical mass. For the last 15 years, he is chairperson of the annual german workshop on polymer analysis by malditof mass spectrometry. Pdf characterization of synthetic polymers via matrix. Purdueuab botanicals center for agerelated disease. Typical applications are synthetic polymers, small peptides, proteins and biopolymers. In principle, ms analysis provides peak masses and then one has to translate those numbers to different polymer species. New sciex api qstar pulsar i maldi head assy 026125. Additionally, by using a solid sample preparation technique, which avoids the concentration gradient problems known to occur with dried droplets and by adding tetraphenylporphyrine as an internal standard to the matrix, it is possible to. Download it once and read it on your kindle device, pc, phones or tablets.
This tutorial demonstrates methods for optimization of the sample preparation, spectral acquisition, and data analysis of synthetic. Originally developed for the analysis of biomolecules, it has developed into one of the most powerful techniques for the characterization of synthetic polymers. Maldi mass spectrometry of synthetic polymers semantic scholar. Agilent g1974a masstech apmaldi pdf ion source for lcmsd trap and tof systems.
In malditof mass spectrometry, the ion source is matrixassisted laser desorptionionization maldi, and the mass. Malditof can analyze a wide range of masses, but is best used for determination of mw 1 kd. If, however, we were to try to analyze a novel synthetic polymer by means of maldims, which to date has not been reported in the literature, we would have to. Use features like bookmarks, note taking and highlighting while reading malditof mass spectrometry of synthetic polymers springer laboratory. Malditof mass spectrometry of synthetic polymers with 158 figures and 45 tables.
An external file that holds a picture, illustration, etc. However, until now these analyses have operated independently, which has resulted in the inability to rapidly connect bacterial phylogenetic identity with potential environmental function. More recently, soft ionization techniques such as electrospray esi and matrix. However, from the ms spectrum alone it is only possible to calculate the sum mass of both end groups. The matrix absorbs energy from the laser and transfers this to the analyte, which is desorbed from the. Current mass spectrometry of synthetic polymer bentham. To date, mass spectrometry ms has been applied to investigate the molecular distribution of cdpr, modifications of cd, and the threaded ratio of cd. Matrixassisted laser desorptionionization timeofflight mass spectrometry malditof ms also allows identification of nocardia at genus level by the analysis of the total proteins ribosomic proteins and proteins from the cell wall. Weidner is the author and coauthor of numerous scientific journal publications and book chapters as well as coeditor of a book. Matrixassisted laser desorptionionization time of flight mass spectrometry malditof ms is a valuable soft ionization analytical technique for characterizing polymers because it can generate. Malditof mass spectrometry ms is set to make inroads into clinical chemistry because it offers advantages over other analytical platforms. In mass spectrometry, matrixassisted laser desorptionionization maldi is an ionization technique that uses a laser energy absorbing matrix to create ions from large molecules with minimal fragmentation.
This standard specifies a general method for determining the average molecular mass and molecular mass distribution of polymers from 2000 g mol 1 to 20000 g mol 1 by matrixassisted laser desorptionionization timeofflight mass spectrometry maldi tof ms. Multidimensional mass spectrometry of synthetic polymers and advanced materials. Quantitation of synthetic polymers using an internal standard by matrixassisted laser desorptionionization timeofflight mass spectrometry. However, only molecular weights mws up to several 10s of kda can be subjected to such analysis, whereas the mw of. Quantitation of synthetic polymers using an internal. An understanding of the parameters that are most influential and the effects of these parameters on the mass spectrum is required for optimization. The malditof ms analysis of polymers can be divided into four distinct steps. Malditof mass spectrometry surface analysis of pet film using a benchtop malditof mass spectrometer laanatme045 malditof mass spectrometers are a mass spectrometer type that is used in a wide range of fields likewise lcms, in terms of high throughput and high sensitivity.
Matrixassisted laser desorptionionization an overview. All major applications of malditof in polymer analysis, including determination of molar mass and analysis of functional groups and copolymers, are presented, and useful information. With the introduction of matrixassistedlaserdesorption ionization timeofflight mass spectrometry malditofms, it has become possible to bring intact polymers in the gas phase. Pdf characterization of synthetic polymers by maldims. Many different polymer applications appeared in recent literature, but most studies deal with homopolymers.
Malditof mass spectrometry is a valuable tool for the analysis of synthetic polymers polymer analysis software can be used to facilitate the interpretation of maldi data derived from polymer. Abstract mass spectrometry of intact synthetic polymers has been limited to field desorption fd ms for many years. For proteinpeptide maldi analysis, most samples are ionized through protonation. Structural analysis of synthetic polymer mixtures using ion mobility and tandem mass spectrometry. Malditof mass spectrometry is one of the latest and most fascinating new developments in the analysis of organic compounds. Analysis of polymers by malditof mass spectrometry. Applications of maldi mass spectrometry in clinical chemistry.
Significant parameters in the optimization of malditofms. He is chairperson of the annual german workshop on the analysis of synthetic polymer by malditof mass spectrometry. Malditof detection of olefin structural isomerization in metathesis chemistry. Analysis of highmolecularweight polyrotaxanes by maldi. In maldims of synthetic polymers, the selection of a matrix and. Matrixassisted laser desorption ionization maldi time of flight tof mass spectrometry is a versatile method used to analyze the composition of biomolecules and other organic macromolecules. Matrixassisted laser desorptionionization wikipedia. The matrixassisted ultraviolet laser desorptionionization timeofflight mass spectra uvmalditofms analysis of some synthetic polymers was studied. This fast and simple technique could be a good choice as a molecular tool for identification at genus level.
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